"Night of the Lizard" is the pilot episode of the animated series Spider-Man produced by John Semper based on the Marvel Comics. It was originally broadcast by the Fox Broadcasting Company on their kids block, "Fox Kids", in the United States on November 19, 1994. This air was months before later episodes from season 1 as a special sneak preview. The pilot introduced the character of Spider-Man as well as various of the shows other major characters, such as his first supervillain in the show, The Lizard. During the episode, the character of Spider-Man comments on how his missions often involve him entering the sewer, when which he references the Fantastic Four, Avengers, Defenders, and the Hulk.
The episode opens with two subway repairmen in conversation. They are then attacked by an over-sized lizard and one of them is kidnapped. Thus, Daily Bugle reports show mysterious disappearances throughout Manhattan and rumors of a giant lizard. Publisher J. Jonah Jameson (Edward Asner) subsequently offers a $1000 bonus to any photographer who can capture an image of the beast. Reporter Peter Parker (Christopher Daniel Barnes), despite originally considering the lizard rumors to be a hoax, plans to claim the reward in order to help pay off some of his Aunt May (Linda Gary)'s large debts. Peter subsequently makes his way to interview his science professor, Dr. Curt Connors (Joseph Campanella), a scientist in which works with reptiles. While paying him a visit, he and fellow science student Debra Whitman (Liz Georges) witness the lizard in their teachers' lavatory. Peter then recalls an experiment he and Dr. Connors worked on which associated with the ability to re-grow mammal limbs using reptilian DNA. Peter furthermore suspects that his science professor is the Lizard. After a confrontation with the beast as Spider-Man (his alter-ego), he confirms this prediction when Curt's wife, Margaret, informs him that her husband was transformed whilst attempting to use his scientific technology to re-grow his arm in which he lost during war. Reporter Eddie Brock (Hank Azaria) eavesdrops on this conversation, leading to Spider-Man webbing him to a lamppost in order to prevent him from revealing the Lizard's identity to the Bugle. Dr. Connors then kidnaps his wife and takes her down into the sewer. He reveals his plan to use the Neogenic Recombinator to transform people of the city into creatures resembling himself. Spider-Man saves him from his condition, however, after firing him with the equipment. Once back at the Daily Bugle, Peter successfully claims the reward and gives the money to his aunt, whilst Eddie Brock is unable to prove the Dr. Connors is the Lizard.