While traveling to the Negative Zone to rescue three children, Spider-Man's suit transformed into a white and black suit. Being that the Negative Zone has no color, the suit made Spider-Man seem almost invisible.
On a second visit to the Negative Zone, Spider-Man gained a version of his red-and-blue nano-tech suit from the TV series Spider-Man Unlimited.
When Spider-Man was framed by Norman Osborn a few weeks after Spider-Man's first Negative Zone visit, he used the costume to become the dark, mysterious Dusk (one of his four new superhero identities during the Identity Crisis story arc). A few months later, after Spider-Man's name was cleared, Cassie St. Commons was given the guise of Dusk and joined the Slingers.
- A Negative Zone alternate costume could be unlocked for those who pre-ordered Spider-Man: Shattered Dimensions at Best Buy. This costume can only be used in the Noir universe.
- In Spider-Man: Edge of Time, if the player has a save file of Spider-Man: Shattered Dimensions on the game system, the costume is unlocked from the start, and wearable for the Amazing Spider-Man dimension/timeline.
- In The Amazing Spider-Man (2012 video game), the Negative Zone costume is wearable after unlocked.
- Cletus Kasady also visited the Negative Zone, finding an exact replica of the Carnage symbiote there, the original one which he had lost when Venom absorbed it into his own symbiote.