The Negative Zone Costume is a costume Spider-Man gained while traveling to the Negative Zone to rescue three children.
During his visit to the Negative Zone, Spider-Man's suit transformed into a white and black suit. Being that the Negative Zone has no color, the suit made Spider-Man seem almost invisible.
When Spider-Man was framed by Norman Osborn a few weeks after Spider-Man's first Negative Zone visit, he used the costume to become the dark, mysterious Dusk (one of his four new superhero identities during the Identity Crisis story arc). A few months later, after Spider-Man's name was cleared, Cassie St. Commons was given the guise of Dusk, and the Negative Zone costume and joined the Slingers.
- Spider-Man: Shattered Dimensions at Best Buy. This costume can only be used in the Noir universe.
- In Spider-Man: Edge of Time, if the player has a save file of Spider-Man: Shattered Dimensions on the game system, the costume is unlocked from the start, and wearable for the Amazing Spider-Man dimension/timeline.
- In The Amazing Spider-Man (2012 video game), the Negative Zone costume is wearable after unlocked.